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21.
血卟啉衍生物(YHpD)合并照光对S180瘤细胞摄取~(86)Rb,瘤细胞的钠泵活性及糖酵解具有明显的抑制作用,且抑制程度随YHpD剂量的增大而增强。瘤细胞的总ATP酶和(Na-K)-ATP酶活性对YHpD的光动力效应也较敏感.YHpD对Mg—ATP酶活性有轻度抑制作用。YHpD并用哇巴因,对瘤细胞摄取~(86)Rb的光动力效应比两种药物单独应用的抑制作用大。 相似文献
22.
对广西武鸣华侨农场4158人进行基线普查,410人进行膳食调查和作8小时夜尿电解质测定,并将高血压低发区广西的资料与高发区的北京作对比.结果显示,体重指数高是高血压一个重要的危险因素,而体重过重又与膳食中摄入碳水化合物和总热量大有关.另一主要的危险因素是钠,膳食中摄入钠高,尿Na和Na/K比值高者,血压水平和高血压的患病率亦高. 相似文献
23.
Takeshi Uchida Yutaka Ohtaki Hideaki Kido Hiroshi Shinyama Kazutaka Hayashi Katsumi Yamanaga Masahiro Watanabe 《Drug development research》1992,26(2):203-212
The diuretic and the antihypertensive actions of torasemide were examined in renal and genetic hypertensive rats and compared to the effects of furosemide. Oral administration of torasemide (1 and 3 mg/kg) elicited a dose-dependent increase in the excretion of urine and electrolytes and elevated the urinary Na/K ratio in both renal and genetic hypertensive rats. Torasemide and furosemide had a similar maximum diuretic effect in the normotensive Wistar rat and the spontaneously hypertensive rat (SHR). However, the diuretic activity of furosemide was weaker in the renal hypertensive rat (RHR). Torasemide showed approximately 30 times greater diuretic potency than furosemide. Torasemide and furosemide demonstrated hypotensive action in hypertensive rat models, but not in the normotensive Wistar rat. Especially in the RHR, torasemide exhibited a more potent hypotensive action than furosemide. These results show that the diuretic and antihypertensive activities of torasemide are effective in various rat models of hypertension, while the diuretic activity of furosemide is weak in certain hypertensive rat models. © 1992 Wiley-Liss, Inc. 相似文献
24.
三七总皂甙(PNS)能抑制心肌总ATP酶活力,但对Na~( )-K~( )-ATP酶无明显影响。三七皂甙单体Rb_1及Rg_1对心肌总ATP酶活力均有抑制作用,但Rb_1的抑制效力显著大于Rg_1·Rb_1能抑制豚鼠离体心房肌的自律性和收缩性,Rg_1也能抑制豚鼠离体心房肌的自律性,但对心房肌的收缩性却无明显影响。提示PNS抑制心肌收缩力这一作用的主要有效成份是Rb_1· 相似文献
25.
体外测定青蒿琥酯钠(SA)对大鼠红细胞膜Na~( )-K~( )-交换ATP酶活性的影响.方法:在反应系统中分别加入不同浓度的SA(0,0.5,1,5和10 μmol·L~(-1)),通过测定反应系统中释放的无机磷含量,计算酶活性.结果:随着SA浓度(O,0.5,1,5和10 μmol·L~(-1))的增高,对Na~( )-K~( )-交换ATP酶活性的抑制作用也随之增强,抑制率分别为15%,29%,46%和75%.将底物ATP浓度增加为125,250,375和500 μmol·L~(-1),进行了酶的动力学测定.用直线回归分析作Eadie-Hofstee动力学曲线,结果表明,SA对该酶的抑制作用为竞争性抑制.结论:提示SA可影响宿主红细胞膜的离子转运及膜的功能. 相似文献
26.
27.
本文对夹竹桃甙抑制Na~+、K~+-ATP酶的动力学作了探讨,并与乌本甙的作用进行了比较。结果表明:夹竹桃甙抑制Na~+、K~+-ATP酶,在Na~+、K~+浓度改变对均为非竞争性抑制,Na~+/K~+比例6:1时,为混合性抑制,而ATP对夹竹桃甙的作用几无影响。 相似文献
28.
《Journal of molecular neuroscience : MN》1996,29(1):67-78
We studied the activity and kinetic parameters of synaptosomal Na, K-ATPase during 15 min of forebrain ischemia and following
60 min of reperfusion produced by reversible common carotid occlusion in Mongolian gerbils. A synaptosomal fraction was obtained
by both differential centrifugation of brain tissue homogenate and centrifugation of crude mitochondrial fraction at a discontinual
sucrose density gradient. We found two components of ATP concentration dependence of ATP hydrolysis that represent two types
of ATP-binding sites: high affinity and low affinity. Neither ischemia nor reperfusion affected kinetic parameters of a high-affinity
site. However, lowaffinity site parameters were affected by both ischemia and ischemia followed by reperfusion. Maximal velocity
(V
max) decreased by 43 and 42% after ischemia and after ischemia/reperfusion, respectively. The apparentK
m for ATP decreased by 52% after ischemia and by 47% after ischemia/reperfusion. The apparent affinities for K+ and Na+ were determined from the ATP hydrolysis rate as a function of Na+ and K+ concentrations. We found the half-maximal activation constant for K+ (K
a
K+) increased by 60% after ischemia and by 146% after ischemia/reperfusion. On the other hand, we found thatK
aNa+ decreased significantly after ischemia/reperfusion (16%). We concluded that it is the dephosphorylation step of the ATPase
reation cycle that is primarily affected by both ischemia and ischemia/reperfusion. This might be caused by alteration of
the protein molecule and/or its surroundings subsequent to ischemia. 相似文献
29.
P Darwin Bell Jean-Yves Lapointe 《Clinical and experimental pharmacology & physiology》1997,24(7):541-547
1. Macula densa (MD) cells are located within the thick ascending limb (TAL) and have their apical surface in contact with tubular fluid and their basilar region in contact with the glomerulus. These cells sense changes in luminal fluid sodium chloride concentration ([NaCl]) and transmit signals resulting in changes in vascular resistance (tubuloglomerular feedback) and renin release. 2. Current efforts have focused on understanding the cellular transport mechanisms of MD cells. Progress in this area has benefited from the use of the isolated perfused TAL-glomerular preparation, which permits direct access to MD cells. 3. Using microelectrodes to measure basolateral membrane potential (VBL) of MD cells, it was found that VBL was very sensitive to changes in luminal fluid [NaCl]. As [NaCl] was elevated from 20 to 150mmol/L, VBL was found to depolarize by over 30 mV. 4. Basolateral membrane potential measurements were also used to identify an apical Na+: 2CI?: K+ cotransport pathway in MD cells that is the major pathway for NaCl entry into these cells. 5. Other work identified a basolateral chloride channel that is presumed to be responsible for changes in VBL during alterations in luminal [NaCl]. This channel, which is the predominant conductance across the basolateral membrane, may be regulated by intracellular Ca2+ and cAMP. 6. An apical Na+: H+ exchanger in MD cells was detected by measuring changes in intracellular pH using the fluorescent probe 2′,7′-bis-(2-carboxyethyl)-5(and-6) carboxyfluorescein. 7. Using patch-clamp techniques, a high density of pH- and Ca2+-sensitive K+ channels was observed at the apical membrane of MD cells. 8. Other studies found that, at the normal physiological conditions prevailing at the end of the TAL (luminal [NaCl] of 20–60 mmol/L), reabsorption mediated by MD cells is very sensitive to changes in luminal [NaCl]. 相似文献
30.
实验性视网膜脱离Na十-K+-转运ATP酶活性及RPE超微结构改变 总被引:2,自引:1,他引:1
目的:探讨实验性视网膜脱离(retinal detachment,RD)后视网膜色素上皮(retinal pigment epithelium,RPE)对视网膜下液(subretinal fluid,SRF)的输导功能。
方法:将制作成功的实验性单眼RD 28只家兔随机分为4组,每组7只,每只兔的另眼作为对照。分别取RD部位的RPE-脉络膜组织和对照眼相应部位的组织匀浆,再测定ATP水解产物无机磷含量,以判断ATP酶活性;并作实验和对照眼的两种组织相应部位的RP正电镜观察。
结果:实验眼Na十-K+-转运ATP酶活性为(2.16士1.26)/μmol(mg·h),对照眼为(4.84±1.59)μmol/(mg·h),二者差异显著(t=5.52,P<0.01);酶活性降低程度随病程延长而减轻(p<0.05)。电镜观察实验眼RD后第2至4周有自脉络膜向视网膜方向的吞饮泡,且随病程延长而增多。
结论;RD发生后RPE的外向输导功能增强,随病程进展,此功能减弱而内向输导功能产生。
(中华眼底病杂志,1997,13:83-85) 相似文献